Search for guide RNA sites in PCR tags

Usage

• Install the package version through pip pip install gRNAsearch
• from grnasearch.gRNA import search
• summary, frames = search(syn="../seq.fa", tag="../tag.csv", save=True, output_dir="~", output_name="gRNA")

Parameters

search(syn, tag, save=True, output_dir='~', output_name="gRNA"):

• syn: full path to sequence file in '.fa' format
• tag: PCR tag file
• save: save the outputs in csv (default=True)
• output_dir: output directory (full path) to save the files (default='~')
• output_name: name of the output file (default="gRNA")

Outputs

• summary: the search results data frame
• frames: the intermediate data frame with WT sequences