Transphire

Cryo-EM on the fly processing pipeline.

If you have any questions contact:
markus.stabrin@mpi-dortmund.mpg.de

External programs

To enjoy the full functionaly of TranSPHIRE, install the following programs:

IMOD 4.9: http://bio3d.colorado.edu/imod
MotionCor2 1.0.0/1.0.5/1.1.0: http://msg.ucsf.edu/em/software/motioncor2.html
SumMovie 1.0.2: http://grigoriefflab.janelia.org/unblur
CTER 1.0: http://sphire.mpg.de
CTFFIND 4.1.8/4.1.10: http://grigoriefflab.janelia.org/ctf
Gctf 1.06,1.18: https://www.mrc-lmb.cam.ac.uk/kzhang

Installation

TranSPHIRE needs an environment that includes Python version 3.6, QT version 5 and PyQt version 5.

Installation using Anaconda

https://www.anaconda.com/download/#linux

Create a new virtual environment

conda create -n transphire python=3.6 pyqt=5

Activate the environment

source activate transphire

Install transphire

pip install transphire

Installation inside your own environment that matches the requirements

Install transphire

pip install transphire

Run TranSPHIRE

Make sure, that you have your transphire environment active. If you start TranSPHIRE for the first time:

transphire --edit_settings

In case the appearing window is too large for your screen, adjust the font size (default 20).
For example:

transphire --edit_settings --font 8

First got to the TranSPHIRE settings tab and after that the Font tab. Adjust the Font value. If necessary, the other options can be adjusted at a later point, after the GUI is opened.

Open the other tabs and adjust the settings according to your system.

Close the dialog and the GUI will open.

In the future, you only need to run:

transphire

If you want to run TranSPHIRE, fill in the tabs from left to right (General to CTF) and press the Start button.

A more detailed tutorial will be available soon.