Restriction Digest Tool

Python/Galaxy tool with the following aims:

• digest DNA into fragments, produce FASTA output of that
• plot restriction digests in easy to use SVG format
• run "hypothetical gels" to help biologists understand the output of these fancy computer things
• optimise restrictions with multiple genomes in order to easily determine enzymes for unique resetriction patterns

Running

python bin/digest_dna.py test.fa Bsp6I,Bsp13I,DseDI

Authors

• Stephen Crosby
• Eric Rasche

Acknowledgements

• Thanks to NEB's REBASE for providing the data backing dnadigest/rebase.yaml.
• Thanks to Wikipedia for their enzyme data backing dnadigest/wikipedia.yaml