pip install pybiotk

git clone https://gitee.com/liqiming_whu/pybiotk.git
cd pybiotk
pip install .

console_scripts =
    gtf2bed = pybiotk.convert.gtf2bed:run
    bed2bedgraph = pybiotk.convert.bed2bedgraph:run
    fq2fasta = pybiotk.convert.fq2fasta:run
    bam2fastx = pybiotk.convert.bam2fastx:run
    bampe_order_by_name = pybiotk.convert.bampe_order_by_name:run
    bam_random = pybiotk.utils.bam_random:run
    gtf_filter = pybiotk.utils.gtf_filter:run
    fasta_filter = pybiotk.utils.fasta_filter:run
    fastq_uniq = pybiotk.utils.fastq_uniq:run
    fastq_join = pybiotk.utils.fastq_join:run
    fastx_rename = pybiotk.utils.fastx_rename:run
    genomefetcher = pybiotk.utils.genomefetcher:run
    reverse_fastx = pybiotk.utils.reverse_fastx:run
    seq_random = pybiotk.utils.seq_random:run
    merge_row = pybiotk.utils.merge_row:run
    read_tables = pybiotk.utils.read_tables:run
    rmats_filter = pybiotk.utils.rmats_filter:run
    count_normalize = pybiotk.utils.normalize:run
    reference_count = pybiotk.utils.reference_count:run
    pyanno = pybiotk.utils.pyanno:run
    rna_fragment_size = pybiotk.utils.fragment_size:run
    merge_subseq = pybiotk.utils.merge_subseq:run
    subseq_analysis = pybiotk.utils.subseq_analysis:run

usage: pyanno [-h] -i INPUT -o OUTPUT -g GTF [-l {transcript,gene}] [--tss_region TSS_REGION [TSS_REGION ...]] [--downstream DOWNSTREAM] [-s]
              [--rule {1+-,1-+,2++,2--,1++,1--,2+-,2-+,+-,-+,++,--}] [-p] [--ordered_by_name]

optional arguments:
  -h, --help            show this help message and exit
  -i INPUT, --input INPUT
                        input file, bam or bed. The file type will be inferred from the filename suffix ['*.bam', '*.bed']. (default: None)
  -o OUTPUT, --output OUTPUT
                        output file name. (default: None)
  -g GTF, --gtf GTF     gtf file download from Genecode, or a sorted gtf file. (default: None)
  -l {transcript,gene}, --level {transcript,gene}
                        annotation level, transcript or gene. (default: transcript)
  --tss_region TSS_REGION [TSS_REGION ...]
                        choose region from tss. (default: [-1000, 1000])
  --downstream DOWNSTREAM
                        downstream length from tes. (default: 3000)
  -s, --strand          require same strandedness. (default: False)
  --rule {1+-,1-+,2++,2--,1++,1--,2+-,2-+,+-,-+,++,--}
                        how read(s) were stranded during sequencing. only for bam. (default: 1+-,1-+,2++,2--)
  -p, --pair            annotate fragments instead of reads. (default: False)
  --ordered_by_name     if input bam is ordered by name, only for pair-end bam. (default: False)