BigphASE

This package was devised to analyze hybrid RNA-seq using bi-parental graph strategy.


License
gnuplot
Install
pip install BigphASE==0.1.2

Documentation

BigphASE

Bi-parental graph ASE analyzer
This python pakage was devised to analyze hybrid RNA-seq using bi-parental graph strategy.
version: 0.1.2
author: Yi Jiang (jiangy296@mail2.sysu.edu.cn); Muran Yi; Zhule Liu

Enviroment required:

xxx

Installation:

`pip install BigphASE`

Usage:

BigphASEtools [-options]

Main steps:

(1) Graph builder Graph genome was built using one parental genome as backbone, with variations between two parental genomes as alternate paths. (2) RNAseq mapping Clean RNA-seq data were mapped to graph index. (3) ASE genes analysis After mapping, reads were assigned to parental origin based on their genotypes at SNP sites, then maternal and paternal origin reads count of each gene were summed.

pipeline